Itgam Mouse Monoclonal Antibody [Clone ID: WT.3]

CAT#: SM264F

Itgam mouse monoclonal antibody, clone WT.3, FITC

Product Images

Staining of rat splenocytes with Mouse anti Rat CD18: FITC

Specifications

Product Data

• Clone Name: WT.3
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10⁶ cells in 100 µl.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: FTL-43. Lymph node cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.
• Specificity: Clone WT.3 reacts with the rat beta 2 integrin, which is designated CD18.
  The beta 2 integrin may be associated with the integrin alphaL, alpha^X or alphaM subunits.
  The antibody has been shown to inhibit homotypic aggregation of PHA blasts.
  It also blocks binding of rat lymphocytes to purified rat ICAM-1.
• Formulation: PBS containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid purified IgG fraction
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit alpha M
• Database Link:
  Entrez Gene 25021 Rat
  UniProt ID Q9JI30
• Background: CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.
• Synonyms: Integrin beta-2, MFI7