CD4 Mouse Monoclonal Antibody [Clone ID: Du CD4-2]

CAT#: SM2271P

CD4 mouse monoclonal antibody, clone Du CD4-2, Purified

Specifications

Product Data

• Clone Name: Du CD4-2
• Applications: FC, IP
• Recommended Dilution: Flow Cytometry.
  Immunoprecipitation.
• Reactivities: Duck
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: 293T cells expressing Pekin duck CD4.
  Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
• Specificity: This antibody recognises CD4.
  Clone Du CD4-2 can be used to identify duck T helper cells.
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID Q50EU7
• Background: CD4 is shown to be expressed by thymocytes, splenocytes and peripheral lymphoid cells. Since the majority of avian immune studies have been carried out on chickens, relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs, the spleen, thymus and bursa of Fabricius. At the cellular level, studies have shown that like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin (PHA), and all cells reacting with clone Du CD4-2 have been identified as CD3+ T cells. (1)
• Synonyms: T4/Leu-3