EMR2 (ADGRE2) Mouse Monoclonal Antibody [Clone ID: 2A1]

CAT#: SM2212R

EMR2 (ADGRE2) mouse monoclonal antibody, clone 2A1, PE

Product Images

Staining of Human peripheral blood granulocytes with Mouse Anti Human CD312 RPE (Cat.-No SM2212RT).

Specifications

Product Data

• Clone Name: 2A1
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10⁶ cells in 100 µl.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA.
  Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
• Specificity: This antibody recognises EMR2.
  Clone 2A1 specifically recognises the stalk region of EMR2.
• Formulation: PBS, pH 7.4
  Label: PE
  State: Lyophilized purified IgG fraction
  Stabilizer: 1% BSA, 5% Sucrose
  Preservative: 0.09% Sodium Azide
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 0.25 ml distilled water.
• Purification: Affinity Chromatography on Protein G
• Conjugation: PE
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
  Should this product contain a precipitate we recommend microcentrifugation before use.
• Stability: Shelf life: one year from despatch.
• Gene Name: adhesion G protein-coupled receptor E2
• Database Link:
  Entrez Gene 30817 Human
  UniProt ID Q9UHX3
• Background: EMR2 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins, which is closely related to CD97. EMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulphate, which binds to the fourth EGF-like module of EMR2.