Itgb1 Hamster Monoclonal Antibody [Clone ID: HM beta 1.1]

CAT#: SM2210R

Itgb1 hamster monoclonal antibody, clone HM beta 1.1, PE

Specifications

Product Data

• Clone Name: HM beta 1.1
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat-1/5 diluted antibody to label 10e6 cells in 100 µl.
  The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
  This may be reduced by appropriate blocking.
• Reactivities: Mouse, Rat
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Purified mouse VLA-4 antigen.
  Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 mouse myeloma cell line.
• Specificity: This antibody recognizes the Integrin beta 1 subunit (CD29).
  Clone HM beta 1.1 is reported to inhibit beta 1 integrin mediated adhesion. (Ref.1)
  We recommend the use of SM2210LE for this purpose.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative, 1% BSA and 5%Sucrose
  Label: PE
  State: Liquid purified IgG fraction
  Label: R. Phycoerythrin (RPE)
• Purification: Affinity Chromatography on Protein G.
• Conjugation: PE
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin beta 1 (fibronectin receptor beta)
• Database Link:
  Entrez Gene 16412 Mouse
  UniProt ID P09055
• Background: CD29 is a 110KD cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. CD29 forms non-covalent bonds with the integrin alpha subunits, including CD51 and CD49a-f, to form heterodimers. The ligands for these heterodimers include collagen, fibronectin, laminin and vascular adhesion molecule-1. In the immune system beta 1 integrins play an important role in cell adhesion, migration, activation and differentiation.
• Synonyms: Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, ITGB1, FNRB, MDF2, MSK12