Thy1 Rat Monoclonal Antibody [Clone ID: FF-10]

CAT#: SM2128P

Thy1 rat monoclonal antibody, clone FF-10, Purified

Specifications

Product Data

• Clone Name: FF-10
• Applications: FC, IHC, IP, WB
• Recommended Dilution: Flow Cytometry (1/50-1/100).
  Immunohistochemistry on Frozen Sections.
  Immunoprecipitation.
  Western Blot.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: EL-4 cells.
  Spleen cells from immunised Sprague-Dawley rats were fused with cells from the mouse SP2/0 - Ag14 myeloma cell line.
• Specificity: This antibody recognises the Thy1.2 cell surface antigen, and binds to T cell lines (EL-4, 3A9), T lymphocytes and the WEH1-3 cell line. 3T3 fibroblasts are also stained by this antibody.
  This clone recognises a 30kD glycoprotein in Immunoprecipitation and Western blotting assays.
  The antibody strongly induces homotypic adhesion of Thy1.2 positive cell lines.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: thymus cell antigen 1, theta
• Database Link:
  Entrez Gene 21838 Mouse
  UniProt ID P01831
• Background: CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.
• Synonyms: Thy-1, THY1, CDw90