Rat Lambda light chain Mouse Monoclonal Antibody [Clone ID: MARL-15]

CAT#: SM2096P

Rat Lambda light chain mouse monoclonal antibody, clone MARL-15, Purified

Specifications

Product Data

• Clone Name: MARL-15
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western Blot: 1/500-1/1000.
  Immunohistochemistry on Frozen Sections.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat lambda light chain.
• Specificity: This antibody recognizes immunoglobulin lambda light chain. This antibody shows no cross-reactivity to Ig heavy chains or to kappa light chain.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).