CD8A Mouse Monoclonal Antibody [Clone ID: 38.65]

CAT#: SM2080R

CD8A mouse monoclonal antibody, clone 38.65, PE

Product Images

Staining of sheep peripheral blood lymphocytes with MOUSE ANTI OVINE CD8:FITC (SM2080R).

Specifications

Product Data

• Clone Name: 38.65
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
• Reactivities: Sheep
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Ovine efferent lymphocytes.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
• Specificity: This anitbody recognises the Ovine CD8 cell surface antigen, which is expressed by the cytotoxic/suppressor subset of T lymphocytes.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative; 5% Sucrose and 1% BSA as stabilizer.
  Label: PE
  State: Lyophilized purified IgG fraction.
  Label: R. Phycoerythrin (RPE)
  Absorption emission: 496 nm / 578 nm
• Reconstitution Method: Restore with 1 ml distilled water
• Purification: Affinity Chromatography on Protein G
• Conjugation: PE
• Storage: Store the antibody undiluted (Prior to and following reconstitution) at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 281060 Bovine
  UniProt ID P31783
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL