c-Myc (MYC) Mouse Monoclonal Antibody [Clone ID: 9E10]

CAT#: SM1863PX

c-Myc (MYC) mouse monoclonal antibody, clone 9E10, Purified

Specifications

Product Data

• Clone Name: 9E10
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: ELISA: 1/100-1/500.
  Western blot: 1/100-1/500 (Non-Reducing Conditions).
  Detects a band of approximately 62kDa in Colo 320HSR cell lysates.
  Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 1x10⁶ cells in 100 µl.
  Membrane permeabilisation is required for this application.
  Immunohistochemistry on Frozen and Paraffin Sections: This product does not require protein digestion pre-treatment of paraffin sections prior to staining. It does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of Human c-Myc.
  Spleen cells from immunised Balb/c mice were fused with cells of the myeloma cell line.
• Specificity: This antibody detects the 62kDa c-myc gene product, which is involved in the regulation of the cell cycle and cell growth.
  This antibody may also be used to detect the commonly used c-Myc 'Tag'.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: v-myc avian myelocytomatosis viral oncogene homolog
• Database Link:
  Entrez Gene 4609 Human
  UniProt ID P01106
• Background: p62c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.
• Synonyms: myc tag, myc-tag, c-myc tag

Reference Data

• Protein Families: Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Stem cell relevant signaling - Wnt Signaling pathway, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway, Thyroid cancer, Wnt signaling pathway