CD146 (MCAM) Rat Monoclonal Antibody [Clone ID: OJ79c]

CAT#: SM1860PS

CD146 (MCAM) rat monoclonal antibody, clone OJ79c, Purified

Specifications

Product Data

• Clone Name: OJ79c
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free antibody for coating.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Western Blot: 0.5-1 µg/ml.
  Immunoprecipitation: 1-2 μg/500 µg protein lysate.
  Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Positive Control: A-375, HUV-EC or HeLa Cells, Tonsil or Melanoma.
• Reactivities: Human
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human MUC18 protein.
• Specificity: This antibody recognises the 118kD cell surface glycoprotein CD146.
  Cellular Localization: Cell surface.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 130 kDa
• Gene Name: melanoma cell adhesion molecule
• Database Link:
  Entrez Gene 4162 Human
  UniProt ID P43121
• Background: The Human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.
• Synonyms: MCAM, MUC18, CD146