LOC100507703 Mouse Monoclonal Antibody [Clone ID: BB7.2]

CAT#: SM1818A

LOC100507703 mouse monoclonal antibody, clone BB7.2, Low Endotoxin

Specifications

Product Data

• Clone Name: BB7.2
• Applications: FC, FN, IHC, IP
• Recommended Dilution: Flow Cytometry: Use 10 µl of 10 µg/ml of diluted antibody to label 10⁶ cells or 100 µl whole blood.
  Immunoprecipitation.
  Functional Assays: 5-10 µg/ml (Inhibition of MHC resticted cytotoxicity).
  Immunohistochemistry on Frozen Sections.
  Recommended Positive Control: Tonsil tissue.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Papain solubilised HLA-A2.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody recognizes the Human HLA-A2 histocompatibility antigen. The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands.
  BB7.2 may be used for the Flow cytometric detection of HLA-A2 expression and has also been used for Immunoaffinity purification of HLA-A2 molecules. Functionally the antibody is reported to inhibit MHC restricted cellular cytotoxicity.
• Formulation: PBS without stabilizer or preservative
  State: Low Endotoxin
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A from tissue culture supernatant.
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P01892
• Background: Human leukocyte antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens, class I (HLA A, HLA B and HLA C) and class II (HLA D).
• Synonyms: HLAA2, MHC class I antigen A-2