Aquaporin 1 (AQP1) (249-269) Mouse Monoclonal Antibody [Clone ID: 1/A5F6]

CAT#: SM1809P

Aquaporin 1 (AQP1) (249-269) mouse monoclonal antibody, clone 1/A5F6, Purified

Specifications

Product Data

• Clone Name: 1/A5F6
• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA: 1/1000-1/20000.
  Western Blot: 1/1000-1/5000.
  Immunoprecipitation: 1/1000-1/10000.
  Immunohistochemistry on Frozen and Paraffin Embedded Sections: 1/500-1/1000.
  This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
  Recommended Positive Control Tissue: Kidney.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to amino acids 249-269 of Rat Aquaporin 1.
  Spleen cells from immunised Balb/c mice were fused with the cells of the Sp2/0 Ag14 mouse myeloma cell line.
• Specificity: This antibody recognises an epitope within the cytoplasmic domain of the water-specific channel Aquaporin 1.
• Formulation: State: Purified
  State: Liquid purified IgG fraction containing 0.09% Sodium Azide as preservative and 0.1% BSA as stabilizer.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: aquaporin 1 (Colton blood group)
• Database Link:
  Entrez Gene 358 Human
  UniProt ID P29972
• Background: Aquaporin 1 is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kD in mass. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
• Synonyms: AQP-1, CHIP28, Aquaporin-CHIP