CD81 / TAPA1 Hamster Monoclonal Antibody [Clone ID: Eat2]

CAT#: SM1526F

CD81 / TAPA1 hamster monoclonal antibody, clone Eat2, FITC

Product Images

Staining of Mouse spleen cells with Hamster Anti Mouse CD81-FITC (SM1526F/FT).

Specifications

Product Data

• Clone Name: Eat2
• Applications: FC
• Recommended Dilution: Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: 38C13, murine B cell line. Spleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.
• Specificity: This antibody recognises and CD81. Eat 2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation. Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding.
• Formulation: PBS, pH 7.4, containing 1% BSA as stabilizer and 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified IgG fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD81 antigen
• Database Link:
  Entrez Gene 12520 Mouse
  UniProt ID P35762
• Background: CD81 is a 26kD cell surface glycoprotein that is also known as TAPA-1. In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation.
• Synonyms: S5.7; TAPA-1; TAPA1; Tetraspanin-28; Tspan-28; TSPAN28