malE Mouse Monoclonal Antibody [Clone ID: R29]
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Specifications
Product Data | |
Clone Name | R29 |
Applications | IP, WB |
Recommended Dilution | Western blot. Immunoprecipitation. |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Recombinant Maltose Binding protein. Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line. |
Specificity | This antibody recognizes maltose binding protein (MBP). The antibody is useful in recognising proteins expressed with an MBP tag. |
Formulation | PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G |
Conjugation | Unconjugated |
Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Background | Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein. |
Synonyms | MMBP, Maltodextrin-binding protein, malE-Tag |
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