ICAM1 Mouse Monoclonal Antibody [Clone ID: 15.2]

CAT#: SM1156PS

ICAM1 mouse monoclonal antibody, clone 15.2, Purified

Product Images

Staining of human peripheral blood monocytes with Mouse Anti Human CD54.

Specifications

Product Data

• Clone Name: 15.2
• Applications: FC, IHC, IP
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/50-1/100 dilution to label 10e6 cells in 100 µl.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections: 1/50-1/100.
  Recommended Positive Control Tissue: Human Tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human monocytes.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/O-Ag14 myeloma cell line.
• Specificity: This antibody recognises the CD54 cell surface antigen.
  Clone 15.2 is reported to block CD54 function.
  We recommend the use of SM1156A for such studies.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: intercellular adhesion molecule 1
• Database Link:
  Entrez Gene 3383 Human
  UniProt ID P05362
• Background: CD54 is a 90kD glycoprotein also known as ICAM-1. CD54 is expressed by many cells following activation by inflammatory mediators.
• Synonyms: ICAM-1