CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: AT10]

CAT#: SM1092R

CD32A (FCGR2A) mouse monoclonal antibody, clone AT10, PE

Product Images

Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD32:RPE (SM1092R).

Specifications

Product Data

• Clone Name: AT10
• Applications: FC
• Recommended Dilution: Flow cytometry: Use 10µl of neat antibody to label 10⁶ cells or 100 µl whole blood.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: K562 cell line.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody recognizes the Human CD32 antigen.
  Clone AT10 blocks the binding of IgG to Fc gamma R11 (See Larsson, M. et al. for details). SM1092LE is recommended for this purpose.
• Formulation: PBS
  Label: PE
  State: Lyophilized purified IgG fraction
  Stabilizer: 1% BSA, 5% sucrose
  Preservative: 0.09% Sodium Azide
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: SM1092R: Restore with 1.0 ml distilled water.
  SM1092RT: Restore with 0.25 ml distilled water.
• Purification: Affinity Chromatography on Protein A
• Conjugation: PE
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: Fc fragment of IgG receptor IIa
• Database Link:
  Entrez Gene 2212 Human
  UniProt ID P12318
• Background: CD32 is a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation.CD32 is expressed by B cells, monocytes, granulocytes and platelets.
• Synonyms: Fc-gamma RII-a, Fc-gamma-RIIa, CDw32, FCGR2A, FCG2, FCGR2A1, IGFR2