Macrophages / Monocytes Rat Monoclonal Antibody [Clone ID: MOMA-2]

CAT#: SM065B

Macrophages / Monocytes rat monoclonal antibody, clone MOMA-2, Biotin

Product Images

Mouse Spleen Frozen Section stained with Biotin conjugated Macrophages / Monocytes Antibody Cat.-NoSM065B

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  Comparison of different mature macrophage markers

Specifications

Product Data

• Clone Name: MOMA-2
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry (Membrane permeabilisation is recommended).
  Immunohistochemistry on Frozen Sections: 0125 μg/ml (1/1600).
  Recommended Positive Control: Mouse spleen.
  Does not work on Formalin-Fixed, Paraffin-Embedded Tissue Sections.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Mouse lymph node stroma.
  Antigen, epitope: The antigen is a (glyco-)protein of 140kDa m.w. which is located within the cytoplasm and on the cell surface. The epitope has not been further characterized.
• Specificity: This antibody clone MOMA-2 is a useful marker for the broad detection of monocytes and macrophages in all mouse strains. In combination with the anti F4/80 marker BM8 it allows a precise characterisation of tissue fixed macrophages in various organs. The antibody stains a mature macrophage subset, monocytes and a few precursors in bone marrow. Dendritic cells show low to intermediate expression. The staining shows close correlation with expression of acid phosphatase in tissue sections.
  MOMA-2 is predominantly expressed in the cytoplasm, but is also present on the cell surface.
  Antigen Distribution
  Isolated Cells: In the cytospin preparation of thioglycollate stimulated peritoneal exudate cells MOMA-2 detects an antigen as distinct cytoplasmic spots. MOMA-2 detects monocytes of the peripheral blood and a subpopulation of bone marrow cells.
  Tissue Sections: MOMA-2 detects typical tissue macrophages as does the anti F4/80-specific clone BM8 (pan macrophage marker). However, different staining patterns are visible as shown below. The most predominant difference can be observed in T-cell areas and follicles of peripheral lymphoid organs where the anti F4/80 clone BM8 is negative.
• Formulation: Stock solution contains PBS, pH 7.2 with 5 mg/ml BSA as a stabilizer and 0.05% (v/v) Kathon as a preservative
  Label: Biotin
  State: Lyophilized purified IgG fraction from cell culture supernatant
• Reconstitution Method: Restore by adding 0.5 ml distilled water (= 0.2 mg/ml Stock Solution).
• Concentration: 0.2 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Synonyms: Macrophage marker, Monocyte marker
• Note: This antibody was produced serum-free, without fetal calf serum.