Thy1 Mouse Monoclonal Antibody [Clone ID: OX-7]

CAT#: SM049F

Thy1 mouse monoclonal antibody, clone OX-7, FITC

Product Images

Staining of rat thymus cells with Mouse anti Rat CD90 (THY-1.1)-FITC

Specifications

Product Data

• Clone Name: OX-7
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 10⁶ cells in 100 µl.
  This product is routinely tested on Rat thymocytes.
• Reactivities: Guinea Pig, Mouse, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat Thy1 antigen.
  Spleen cells of immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody recognises CD90, also known as Thy1.1. Since Thy1.1 is a monomorphic determinant in Rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c.
  Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic neurons, using Fluorescence Microscopy (See Jeng, C .J. et al. for details).
  The Affinity of the Fab' of MRC OX-7 for Rat Thy1 is 3 x 10⁹m^-1 and Mouse Thy1.1 is 3 x 10⁸m^-1 (See Mason, D.W. & Williams, A.F. for details).
  This clone has been reported to induce glomerular nephritis in Wistar rats (See Tamura, M. et al. for details).
• Formulation: PBS
  Label: FITC
  State: Liquid purified IgG fraction.
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
  Label:

  Fluorescein Isothiocyanate Isomer 1 (Excitation / Emission: 525 nm / 490 nm)

• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Thy-1 cell surface antigen
• Database Link:
  Entrez Gene 24832 Rat
  UniProt ID P01830
• Background: Thy1.1 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues.
• Synonyms: Thy-1, THY1, CDw90