Cd40 Rat Monoclonal Antibody [Clone ID: 3/23]

CAT#: SM008P

Cd40 rat monoclonal antibody, clone 3/23, Purified

Product Images

Staining of mouse spleen cells with Rat anti Mouse CD40

Specifications

Product Data

• Clone Name: 3/23
• Applications: ELISA, FC, IHC
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl.
  Immunohistology on Frozen Sections.
  ELISA.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1.
  Spleen cells from immunised LOU/c rats were fused with cells of the Ag8 mouse myeloma cell line.
• Specificity: Specificity was checked by ELISA and flow cytometry using BHK cells transfected with mouse CD40.
  It does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells.
  It does not cross react with mouse T cells.
  Clone 3/23 is a powerful activator of normal B cells especially in the presence of IL-4.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD40 antigen
• Database Link:
  Entrez Gene 21939 Mouse
  UniProt ID P27512
• Background: CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.
• Synonyms: TNFRSF5, CDw40, Bp50