Pyranose Oxidase Goat Polyclonal Antibody

CAT#: R1156PS

Pyranose Oxidase goat polyclonal antibody, Purified

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Goat anti-Pyranose oxidase antibody ([R1165P]; lot 8178) was used to detect Pyranose oxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1g of pr

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot : 1/500 - 1/5,000.
  Immunoprecipitation: 1/100.
  ELISA : 1/5,000 - 1/20,000:
  This product has been assayed against 1.0 µg of Pyranose oxidase [E.coli] in a standard ELISA using peroxidase conjugated affinity purified anti-goat IgG and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested.
• Reactivities: Escherichia coli
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Pyranose oxidase from E.coli
• Specificity: This antibody detects Pyranose oxidase from E. coli. Cross reactivity against Pyranose oxidase from other microbes is known but not tested
  Iimmunoelectrophoresis give a single precipitin arc against anti-goat serum as well as purified and partially purified Pyranose oxidase [E.coli].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  State: Purified
  State: Lyophilized purified Ig fraction
  Preservative: 0.01% (w/v) Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.