OVAL Rabbit Polyclonal Antibody

CAT#: R1101HRPS

OVAL rabbit polyclonal antibody, HRP, Purified

Product Images

Western blot of rabbit anti-Ovalbumin peroxidase conjugated antibody. Lane 1: Ovalbumin (hen egg). Load: 50 ng per lane. Primary antibody: Rabbit anti-Ovalbumin peroxidase conjugated antibody at 1/1,000 overnight at 4°C. Secondary antibody: none. Blocking buffer for 30 min at RT. Predicted/observed size: 43 kDa for Ovalbumin. Other band (s): Ovalbumin splice variants and isoforms.

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  Western blot of rabbit anti-Ovalbumin peroxidase conjugated antibody. Lane 1: Ovalbumin. Load: 50 ng per lane. Primary antibody: Rabbit anti-Ovalbumin peroxidase conjugated antibody at 1/1,000 overnight at 4°C. Secondary antibody: none. Blocking buffer for 30 min at RT. Predicted/observed size: 43 kDa for Ovalbumin. Other band (s): Ovalbumin splice variants and isoforms.

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA: 1/5,000-1/25,000.
  Western blot: 1/1,000-1/5,000.
  Immunohistochemistry: 1/500-1/2,500.
  Immunoprecipitation: 1/100.
• Reactivities: Chicken
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Native Ovalbumin from hen egg white
• Specificity: This antibody detects Chicken (Gallus gallus) Ovalbumin. Cross reactivity against Ovalbumin from other tissues and species may occur but have not been specifically determined.
  Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Ovalbumin [hen egg white].
• Formulation: 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified IgG fraction
  Stabilizer: 10 mg/ml BSA (immunoglubulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ovalbumin (SERPINB14)
• Database Link:
  Entrez Gene 396058 Chicken
  UniProt ID P01012
• Background: Chicken Ovalbumin is the major protein in the "white" of the egg (and a favorite antigen in immunological research). Egg white contains a variety of proteins including ovalbumin, conalbumin, ovomucoid and lysozyme. It belongs to the serpin family and the Ov serpin subfamily. Ovalbumin can cause an allergic reaction in humans. Ovalbumin has been implicated in the development of the egg shell. Immunohistochemistry revealed that ovalbumin is found only in the mammillary bodies of decalcified shell, and is not distributed throughout the shell matrix. These results indicate that ovalbumin is present during the initial phase of shell formation and becomes incorporated into the protein matrix of the mammillary bodies. However, it is not yet clear whether ovalbumin at this site plays a specific role in shell mineralisation.
• Synonyms: SERPINB14, Egg albumin, Plakalbumin, Allergen Gal d II