GOT1 Sheep Polyclonal Antibody

CAT#: R1087

GOT1 sheep polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most immunological methods requiring high titer and specificity.
  Recommended Dilutions:
  ELISA: 1/5,000-1/25,000.
  Western Blot: 1/500-1/3,000.
  This product has been assayed against 1.0 µg of Aspartate Aminotransferase (AST) [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified Goat anti-Sheep IgG [H&L] (R1364HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
  A working dilution of 1/4,000 to 1/16,000 of the reconstitution concentration is suggested for this product.
• Reactivities: Porcine
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Aspartate Aminotransferase (AST) from Porcine Heart.
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart].
  Cross reactivity against Aspartate Aminotransferase (AST) from other tissues and species may occur but have not been specifically determined.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide as preservative.
  State: Serum
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Prepared from monospecific antiserum by a Delipidation and Defibrination.
• Conjugation: Unconjugated
• Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below.
  This product is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  UniProt ID P00503
• Background: Aspartate Aminotransferase [Glutamate oxaloacetate transaminase] is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene.
  Serum GOT [with SGPT] levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.
• Synonyms: Aspartate aminotransferase, Transaminase A
• Note: Aspartate Aminotransferase (AST) is also referred to as Glutamic Oxaloacetic Transaminase (GOT).