Carbonic Anhydrase I (CA1) Goat Polyclonal Antibody

CAT#: R1067PS

Carbonic Anhydrase I (CA1) goat polyclonal antibody, Purified

Product Images

Western blot of goat anti Carbonic anhydrase I antibody. Lane 1: Carbonic anhydrase I. Load: 50 ng per lane. Primary antibody: Carbonic anhydrase I antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP goat secondary antibody at 1/40,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic anhydrase I.

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot: 1/500-1/2,000.
  Immunoprecipitation: 1/100.
  ELISA: 1/2,000-1/20,000.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Carbonic Anhydrase I from Human erythrocytes
• Specificity: This product detects human Carbonic anhydrase I. Cross reactivity against Carbonic anhydrase I from other tissues and species may occur but have not been specifically determined.
  Immunoelectrophoresis give a single precipitin arc against anti-goat serum as well as purified and partially purified Carbonic anhydrase I [Human Erythrocytes].
  
  
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  State: Purified
  State: Lyophilized purified Ig fraction
  Preservative: 0.01% (w/v) sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: carbonic anhydrase 1
• Database Link:
  Entrez Gene 759 Human
  UniProt ID P00915
• Background: Carbonic Anhydrase I (CAI) is a single polypeptide chain metalloenzyme of molecular weight 29 kDa. It is present mainly in erythrocytes at a concentration of about 12 mg per gram haemoglobin. Its main enzyme function is to catalyse the reversible hydration of carbon dioxide. It also catalyses the hydrolysis of certain esters. The twelve active CA isozymes are thought to regulate a variety of cellular functions including several processes in the reproductive systems. A mutational deficiency has been described but patients exhibit no adverse chemical symptoms. Red cell CAI levels are decreased in patients with thyrotoxicosis.
• Synonyms: Carbonic anhydrase I, Carbonate dehydratase I, Carbonic anhydrase B, CA1, CAB, CA-I