Bilirubin oxidase Goat Polyclonal Antibody

CAT#: R1066PS

Bilirubin oxidase goat polyclonal antibody, Purified

Product Images

Rockland goat anti Bilirubin oxidase antibody Cat.-No. [R1066P] (Lot 6533) was used to detect Bilirubin oxidase under reducing (R) conditions. A samples of ~1g of purified protein contained 4% BME and was boiled for 5 minutes and run by SDS-PAGE on a 4-20% a

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot: 1/500-1/5,000.
  Immunoprecipitation: 1/100.
  ELISA: 1/5,000-1/20,000.
  This product has been assayed against 1.0 µg of Bilirubin oxidase [Myrothecium verrucaria] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-goat IgG (goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested.
• Reactivities: Myrothecium
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Bilirubin oxidase from Myrothecium verrucaria
• Specificity: This antibody detects Bilirubin oxidase [Myrothecium verrucaria]. Cross reactivity against Bilirubin oxidase from other sources is unknown.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum as well as purified and partially purified Bilirubin oxidase [Myrothecium verrucaria].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  State: Purified
  State: Lyophilized purified Ig fraction
  Preservative: 0.01% (w/v) Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID Q12737
• Background: Bilirubin Oxidase is an enzyme that catalyses the oxidation of bilirubin to biliverdin and other substrates. This enzyme has been expected to be used in the field of clinical pathology for the enzymatic determination
  of total bilirubin in serum and the treatment of jaundice and hyperbilirubinemia.