Collagen III (COL3A1) Rabbit Polyclonal Antibody

CAT#: R1040B

Collagen III (COL3A1) rabbit polyclonal antibody, Biotin

Product Images

Immunohistochemistry-Paraffin Sectiosn Collagen III Antibody Biotin conjugated. This antibody showed strong staining in FFPE sections of human skin (left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
  Recommended Dilutions:
  ELISA: 1/10,000-1/50,000.
  Western blot: 1/1,000-1/5,000.
  Immunoprecipitation: 1/100.
  Immunohistochemistry: 1/200 -1/1,000.
• Reactivities: Bovine, Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Collagen Type III from Human and Bovine placenta.
• Specificity: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
  Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
  This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V and VI collagens.
  Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
• Formulation: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction
  Stabilizer: 10 mg/ml BSA (Ig and Protease free)
  Preservative: 0.01% (w/v) Sodium Azide
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
• Reconstitution Method: Restore with 100 µl deionized water (or equivalent)
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Biotin
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: collagen type III alpha 1 chain
• Database Link:
  Entrez Gene 1281 Human
  UniProt ID P02461
• Background: Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
• Synonyms: COL3A1