beta Defensin 3 (DEFB103A) Rabbit Polyclonal Antibody

CAT#: PP1229B2

beta Defensin 3 (DEFB103A) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human BD-3 by Direct ELISA (using 100µl/well) a concentration of ~1.0 µg/ml of this antibody is required. This Biotinylated antibody allows the detection of at least 0.2-0,4 mg/well of recombinant Humand BD-3.
  Sandwich ELISA: To detect Human BD-3 by Sandwich ELISA (using 100µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. This Biotinylated antibody, in conjugation with Purified Anti-Human BD-3 antibody (Cat.-No PP1229P) as a Capture antibody, allows the detection of at least 0.2-0.4 mg/well of recombinant Humand BD-3.
  Western blot: To detect Human BD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human BD-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: E.coli derived recombinant Human Beta Defensin-3 (Cat.-No PA184).
• Specificity: This antibody reacts with Human BD3 (Defensin beta 3). Other species not tested.
• Formulation: BPS, pH 7.2 without preservatives
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening.
  Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: defensin beta 103A
• Database Link:
  Entrez Gene 414325 Human
  UniProt ID P81534
• Background: Beta Defensin 3 exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. It kills multiresistant S.aureus and vancomycin-resistant E.faecium, but it appears to have no significant hemolytic activity. Beta Defensin 3 is highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression is observed in salivary gland, bone marrow, colon, stomach, polyp and larynx, with no expression in small intestine. Expression is induced by infection of bacteria and by interferon gamma.
  Beta Defensin 3 belongs to the beta-defensin family.
• Synonyms: DEFB103A, DEFB103B, BD3, DEFB3, HBP3, HBD3, DEFB103, Beta-defensin 103, Defensin-like protein, Defensin beta 103
• Note: Molecular weight: ~150 kDa