BAFF (TNFSF13B) Goat Polyclonal Antibody

CAT#: PP1203B1

BAFF (TNFSF13B) goat polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human BAFF by Direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hBAFF.
  Sandwich ELISA: To detect Human BAFF by sandwich ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 µg/ml. This biotinylated polyclonal antibody, in conjunction with Poyclonal Anti-Human BAFF (PP1203P) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human BAFF.
  Western Blot: To detect Human BAFF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human BAFF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Human BAFF.
• Specificity: This antibody recognizes Human BAFF.
  Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized (Sterile filtered) purified IgG fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: tumor necrosis factor superfamily member 13b
• Database Link:
  Entrez Gene 10673 Human
  UniProt ID Q9Y275
• Background: Members of the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL1 (for TNF- and ApoL- related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NFkB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell stimulator since it induced B cell proliferation and immunoglobulin secretion. Two receptors for BAFF were recently identified and designated TACI and BCMA. BAFF also signals through a third TNF receptor BAFFR/BR3. BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B cell associated autoimmune diseases. Like TNFa and TRAIL, THANK was shown to activate NF-kB and c-jun N terminal kinase (JNK) and to induce apoptosis.
  The human BAFF gene codes for a 285 amino acid type II transmembrane protein containing a 46 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 218 amino acid extracellular domain.
• Synonyms: TNFSF13B, BLYS, TALL1, TNFSF20, ZTNF4