DEFB4B Goat Polyclonal Antibody

CAT#: PP1125P2

DEFB4B goat polyclonal antibody, Aff - Purified

Product Images

Sandwich ELISA using Defensin beta 2 Antibody Cat.-No [PP1125P]

Specifications

Product Data

• Applications: ELISA, Neutralize, WB
• Recommended Dilution: Sandwich ELISA: To detect Human BD-2 by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with biotinylated Anti-Human BD-2 (Cat.-No PP1125B1 or PP1125B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human BD-2.
  Western Blot: To detect Human BD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
  Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human BD-2 (1.0 μg/ml), a concentration of 5.0-8.0 mg/ml of this antibody is required.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Human BD-2 (Cat.-No PA139)
• Specificity: This antibody recognizes Human BD2 (Defensin beta 2).
  Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified fraction
• Reconstitution Method: Centrifuge vial prior to opening.
  Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Immunoaffinity Chromatography employing immobilized hBD-2 matrix.
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: defensin beta 4B
• Database Link:
  Entrez Gene 100289462 Human
  UniProt ID O15263
• Background: This antibiotic peptide is locally regulated by inflammation. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence.
  hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.
• Synonyms: Beta-defensin 4A, Beta-defensin 2, BD-2, hBD-2, DEFB102, DEFB2, Skin-antimicrobial peptide 1, DEFB4B, DEFB4A
• Note: Molecular Weight: ~150 kDa