Il12a (alpha + beta) Goat Polyclonal Antibody

CAT#: PP1110P1

Il12a (alpha + beta) goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-12 (0.3 ng/ml), a concentration of 0.012 - 0.2 µg/ml of this antibody is required.
  ELISA:To detect mIL-12 by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant mIL-12.
  Western Blot: To detect mIL-12 alpha by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-12 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
  Immunohistochemistry: We recommend use of 1 µg per ml as a starting dilution. Paraffin sections may require pretreatment like Proteinase K or Trypsin predigestion to improve staining.
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant mouse IL-12
• Specificity: Interleukin-12 (IL-12)
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml
• Purification: Affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: interleukin 12a
• Database Link:
  Entrez Gene 16159 Mouse
  UniProt ID P43431
• Background: Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10; homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.
• Synonyms: IL-12A, IL-12 subunit p35, NKSF1, CLMF p35
• Note: Centrifuge vial prior to opening!