Cxcl10 Rabbit Polyclonal Antibody

CAT#: PP1083P2

Cxcl10 rabbit polyclonal antibody, Aff - Purified

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To detect mIP-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with the Biotinylated Anti-Murine IP-10 PP1083B1/PP1083P2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIP-10.

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Mouse IP-10 (100 ng/ml), a concentration of 10.0 µg/ml of this antibody is required.
  Sandwich ELISA: To detect Mouse IP-10 by Sandwich ELISA (using 100 µl/well antibody solution), a concentration of 0.5-2.0 µg/ml of this antibody is required. This Affinity purified antibody, in conjuction with Biotin conjugated anti Mouse IP10 antibody (cat. PP1083B) as a Detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse IP-10.
  Western Blot: To detect Mouse IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Mouse IP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Murine IP-10.
• Specificity: Reacts with Interferon-gamma Inducible Protein.
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography using an immobilized Murine IP-10 Matrix
• Conjugation: Unconjugated
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: chemokine (C-X-C motif) ligand 10
• Database Link:
  Entrez Gene 15945 Mouse
  UniProt ID P17515
• Background: Interferon-gamma-inducible 10 kD protein (IP-10), is a CXC chemokine with chemoattractant properties for CD4-positive T cells and inhibits early normal and leukemic hemopoietic progenitor proliferation. IP-10 is produced by a wide variety of cell types ranging from neutrophils and monocytes to hepatocytes, endothelial cells and keratinocytes. The cytokine is reported to be involved in a scala of inflammatory pathologies such as HIV encephalitis, cutaneous T cell lymphoma, chronic hepatitis and acute anterior uveitis. Various observations strongly suggest a role for the CXC chemokines IL-8 and IP-10 in the regulation of angiogenic activity in cancer and in idiopathic pulmonary fibrosis.
• Synonyms: C-X-C motif chemokine 10, Small-inducible cytokine B10, Gamma-IP10, INP10, SCYB10
• Note: Centrifuge vial prior to opening!