Thrombopoietin (THPO) Goat Polyclonal Antibody

CAT#: PP1074P2

Thrombopoietin (THPO) goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTPO (5.00 ng/ml), a concentration of 4.2-6.3 µg/ml of this antibody is required.
  ELISA:
  (Indirect): To detect hTPO using 100 μl/well antibody solution. A concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO.
  (Sandwich): To detect hTPO using 100 μl/well antibody solution, a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TPO (PP1074B) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO.
  Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly purified recombinant human TPO.
• Specificity: This antibody reacts with Thrombopoietin (TPO).
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: thrombopoietin
• Database Link:
  Entrez Gene 7066 Human
  UniProt ID P40225
• Synonyms: THPO, MGDF, C-mpl ligand
• Note: Centrifuge vial prior to opening!