M-CSF (CSF1) Rabbit Polyclonal Antibody

CAT#: PP1048P2

M-CSF (CSF1) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry: This M-CSF antibody stained formalin-fixed, paraffin embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

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Specifications

Product Data

• Applications: ELISA, FN, IHC, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hM-CSF (7.0 ng/ml), a concentration of 0.02-0.03 µg/ml of this antibody is required.
  ELISA:
  (Indirect): To detect hM-CSF by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hM-CSF.
  (Sandwich): To detect hM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human M-CSF (PP1048B) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hM-CSF.
  Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
  Immunohistochemistry: 0.5 µg/ml with an overnight incubation at 4°C. This antibody stained Formalin-Fixed, Paraffin-Embedded sections of Human cervical squamous cell carcinoma. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant hM-CSF (human M-CSF).
• Specificity: Human Macrophage Colony Stimulating Factor (M-CSF)
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: colony stimulating factor 1
• Database Link:
  Entrez Gene 1435 Human
  UniProt ID P09603
• Synonyms: CSF1, MCSF, Macrophage colony-stimulating factor 1, Macrophage Colony Stimulating Factor
• Note: Centrifuge vial prior to opening!