Interferon gamma (IFNG) Rabbit Polyclonal Antibody

CAT#: PP1019P2

Interferon gamma (IFNG) rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot unreduced using IFNG / Interferon gamma Antibody Cat.-No [PP1019P].

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  Sandwich ELISA using IFNG / Interferon gamma Antibody Cat.-No [PP1019P].

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  Immunohistochemistry The Interferon gamma antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.5 ug/ml-0.25ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

Specifications

Product Data

• Applications: ELISA, FN, IHC, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of h-IFN-gamma (10 ng/ml), a concentration of 0.13-0.20 µg/ml of this antibody is required.
  Sandwich ELISA: To detect Human IFN-gamma by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotin conjugated IFN-gamma antibody (Cat.-No PP1019B1 or PP1019B2) as detection antibody allows the detection of 0.2-0.4 ng/well of recombinant Human IFN-gamma.
  Western blot: To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
  Immunohistochemistry: Formalin-fixed, paraffin-embedded sections. The recommended concentration is 0.5-0.25 µg/ml with an overnight incubation at 4°C. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly purified (>98%) E.coli derived recombinant Human Inferferon gamma (Cat.-No PA070)
• Specificity: This antibody reacts with Human Interferon gamma (hIFN-gamma).
  Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography using immobilized Human IFN-gamma matrix
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: interferon, gamma
• Database Link:
  Entrez Gene 3458 Human
  UniProt ID P01579
• Background: Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
• Synonyms: IFN-gamma, gamma IFN
• Note: Centrifuge vial prior to opening!