6Ckine / CCL21a Rabbit Polyclonal Antibody

CAT#: PP021P2

6Ckine / CCL21a rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mExodus-2 (100.0 ng/ml), a concentration of 9.0-10.0 µg/ml of this antibody is required.
  Direct ELISA: To detect mExodus-2 by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant mExodus-2.
  Sandwich ELISA: To detect mExodus-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine Exodus-2 (Cat.-No PP021B) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mExodus-2.
  Western blot: To detect mExodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mExodus-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Mouse Exodus-2 (Cat.-No PA003)
• Specificity: This antibody recognises Mouse Exodus-2.
  Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography employing immobilized mExodus-2 matrix
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: C-C motif chemokine 21c
• Database Link:
  Entrez Gene 100041504 Mouse
  UniProt ID P86792
• Background: Exodus 2 is one of several CC cytokine genes clustered on the p arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterised by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).
• Synonyms: C-C motif chemokine 21a, Beta chemokine exodus-2, Small-inducible cytokine A21a, Scya21a, TCA4, TCA-4
• Note: Centrifuge vial prior to opening!