IL2 Mouse Monoclonal Antibody [Clone ID: 1H10-1-1]

CAT#: PM1000A

IL2 mouse monoclonal antibody, clone 1H10-1-1, Azide Free

Product Images

Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.5-5.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

Specifications

Product Data

• Clone Name: 1H10-1-1
• Applications: ELISA, IHC, WB
• Recommended Dilution: Sandwich ELISA: In a Sandwich ELISA (assuming 100 µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human IL-2 when used with Biotin conjugated anti-Human IL-2 (Cat.-No PP1024B) as the detection antibody at a concentration of approximately 1.0-2.0 µg/ml.
  Western Blot: To detect human IL-2 by Western blot analysis this antibody can be used at a concentration of 1.0-2.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
  Immunohistochemistry on Paraffin Sections: 2.5-5.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium citrate buffer is recommended.
  Positive Control: Human Breast invasive ductal carcinoma.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified (>98%) E.coli derived recombinant Human IL-2 (Cat.-No PA075)
• Specificity: Detects Human Interleukin 2. Other species not tested.
• Formulation: PBS without preservatives
  State: Azide Free
  State: Lyophilized (Sterile filtered) purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution the antibody can be stored at 2-8°C for two weeks
  or (in aliquots) at -20°C for at least 6 months.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: One year from despatch.

• Gene Name: interleukin 2
• Database Link:
  Entrez Gene 3558 Human
  UniProt ID P60568
• Background: Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.
• Synonyms: IL-2, TCGF