Histamine Rabbit Polyclonal Antibody

CAT#: EUD801

Histamine rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Immunofluorescence: 1/300-1/600 overnight at 2-8°C.
  Immunohistochemistry on Frozen and Paraffin Sections: 1/1500-1/2000 (PAP).
  Positive Control: Cryostat sections of carbodiimide fixed Human skin or freeze-dried paraffin-sections (vapor fixed in diethylpyrocarbonate, DEPC) of Rat stomach.
• Reactivities: Human, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Histamine conjugated to HSA.
• Specificity: This antibody recognizes Histamine.
  Absorption with 10-100 µg Histamine per ml diluted antiserum abolishes the staining, while Nordrenaline, 5-HT, VIP, Glucagon and Histidine do not.
• Formulation: State: Serum
  State: Lyophilized Undiluted Serum
• Reconstitution Method: Dissolve the antiserum in 50-100 µl distilled water, and dilute further in 0.1M PBS with 1% BSA and 0.09% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Background: Histamine is a neurotransmitter in the central nervous system a well as a mast cell constituent. In addition, histamine is produced by endocrine cells (ECL-cells) in the oxynthic mucosa of the stomach.