Vegfa Rabbit Polyclonal Antibody

CAT#: DP3520B

Vegfa rabbit polyclonal antibody, Biotin, Aff - Purified

Product Images

VEGF-A Sandwich-ELISA using the Biotinylated polyclonal anti-Mouse VEGF-A antibody for detection and recombinant Human VEGF165 ( DA3514X) and Mouse VEGF164 ( DA3518X) as standard. A unbiotinylated Rabbit anti-Mouse VEGF-A antibody was used as capture antibody.

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: ELISA: 1-10 µg/ml.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (> 95%) recombinant Murine VEGF164 Dimer derived from insect cells
• Specificity: The antibody recognizes VEGF-A.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Aff - Purified
  State: Lyophilized purified IgG fraction
  Stabilizer: 50x BSA
  Preservative: 0.02% Sodium Azide
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml. Centrifuge vial prior to opening.
• Purification: Antigen Affinity Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: vascular endothelial growth factor A
• Database Link:
  Entrez Gene 22339 Mouse
  UniProt ID Q00731
• Background: Mouse Vascular Endothelial Growth Factor164 (VEGF164), a 24 kDa protein consisting of 164 amino acid residues, is produced as a homodimer. VEGF164 is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF164 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (Flk-1). In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo and is also a chemoattractant for monocytes and endothelial cells. At least three different proteins are generated by differential splicing of the mouse VEGF gene: VEGF120, VEGF164 and VEGF188. The most abundant form is VEGF164. Whereas VEGF120 and VEGF164 are secreted proteins, VEGF188 is strongly cell-associated. In addition, the isoforms VEGF164 and VEGF188 bind to heparin with high affinity. A related protein of VEGF is placenta growth factor (PlGF) with about 53% homology and VEGF-B with similar biological activities.
• Synonyms: VEGFA, VEGF, VPF, Vascular endothelial growth factor A, Vascular permeability factor