ALK Mouse Monoclonal Antibody [Clone ID: 5A4]

CAT#: DM416

ALK mouse monoclonal antibody, clone 5A4, Supernatant

Product Images

Figure 1. Formalin-fixed, paraffin-embedded human anaplastic lymphoma stained with p80/ALK Ab (Cat. DM416) using peroxidase-conjugate and AEC chromogen. Note nuclear and cytoplasmic staining of tumor cells.

Specifications

Product Data

• Clone Name: 5A4
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Frozen and Paraffin Sections (ABC method: 1/25-1/70).
  High temperature treatment of formalin-fixed tissue sections with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining.
  Incubation Time: 60 minutes at room temperature.
  Positive Control: Anaplastic lymphoma.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Prokaryotic recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of NPM-ALK transcript (419-520 aa).
• Specificity: Recognizes ALK/p80.
  Cellular Localization: Cytoplasmic and/or nuclear.
• Formulation: State: Supernatant
  State: Tissue Culture Supernatant
• Purification: < 0.1 Sodium Azide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: anaplastic lymphoma receptor tyrosine kinase
• Database Link:
  Entrez Gene 238 Human
  UniProt ID Q9UM73
• Background: Anaplastic large cell lymphoma (ALCL) is usually composed of large pleomorphic cells, which express CD30 antigen and the epithelial membrane antigen (EMA). These tumor cells tend to occur in younger patients and may be associated with cutaneous and extranodal involvement. Large cell lymphoma account for approximately 25 % of all non-Hodgkin's lymphomas in children and young adults.
• Synonyms: ALK tyrosine kinase receptor