EPCAM Mouse Monoclonal Antibody [Clone ID: Ber-EP4]

CAT#: DM406

EPCAM mouse monoclonal antibody, clone Ber-EP4, Supernatant

Product Images

DM406 clone Ber-EP4 Ep-CAM Antibody Staining of Formalin-Fixed, Paraffin-Embedded Human breast carcinoma using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of tumor cells.

Specifications

Product Data

• Clone Name: Ber-EP4
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use at 1/50-1/150 dilution in an ABC method (30 minutes at RT).
  Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
  Recommended Positive Control: Kidney.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: BALB/C mice were immunized with human breast carcinoma cell line, MCF-7.
• Specificity: Recognizes Human Epithelial Antigen. Other species not tested.
  Cellular Localization: Cell membrane.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant
  Preservative: Less than 0.1% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: epithelial cell adhesion molecule
• Database Link:
  Entrez Gene 4072 Human
  UniProt ID P16422
• Background: Epithelial antigen is cell surface glycoprotein and is broadly distributed in epithelial cells and displays a highly conserved expression in carcinomas. Epithelial antigen has been know to play an important role as tumor-cell marker in lymph nodes from patients with esophageal carcinoma. Epithelial antigen can be used to distinguish between basal cell and basosquamous carcinomas and squamous cell carcinoma of the skin.
• Synonyms: Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2, M4S1, MIC18