GFAP Mouse Monoclonal Antibody [Clone ID: 6F2]

CAT#: DM199P

GFAP mouse monoclonal antibody, clone 6F2, Purified

Specifications

Product Data

• Clone Name: 6F2
• Applications: IHC, WB
• Recommended Dilution: Immunoblotting.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections.
  Recommended Dilutions: 1/25–1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for Immunoblotting applications.
  Recommended Positive Control: Brain.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Glial Fibrillary Acidic Protein from Human brain.
• Specificity: This antibody 6F2 reacts exclusively with glial fibrillary acidic protein which is present in astrocytes in the central nervous system and Schwann cells.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freeze-thaw cycles.
• Stability: Shelf life: One year from despatch.
• Gene Name: glial fibrillary acidic protein
• Database Link:
  Entrez Gene 2670 Human
  UniProt ID P14136
• Background: GFAP (55 kD) is selectively located in astrocytes and represents the major constituent of astrocytic intermediate filaments. GFAP expression levels are highly variable during development of the central nervous system. In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimer's disease, epilepsy and multiple sclerosis. In the peripheral nervous system GFAP is expressed by Schwann cells. Upon differentiation, myelin forming Schwann cells down-regulate GFAP, whereas in non-myelin forming Schwann cells GFAP persists into adulthood.
• Synonyms: Glial Fibrillary Acidic Protein