CCR4 Mouse Monoclonal Antibody [Clone ID: KH-4F5]

CAT#: DM1232

CCR4 mouse monoclonal antibody, clone KH-4F5, Purified

Product Images

Figure 4: SDS-PAGE analysis of purified KH-4F5 monoclonal antibody. Lane 1: Molecular weight marker, Lane 2: 2 ug of purified KH-4F5 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.

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  Figure 2: Spectral Confocal Microscopy of CHO cells using KH-4F5 antibody. CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of KH-4F5 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).

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  Figure 1: FACS analysis of BOSC23 cells using KH-4F5 antibody. BOSC23 cells were transiently transfected with an expression vector encoding either CCR4 (Red curve) or an irrelevant protein (control transfectant). Binding of KH-4F5 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CCR4 transfected cells.

Specifications

Product Data

• Clone Name: KH-4F5
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
  Flow cytometry: 1.2 µg/10⁶ cells.
  Immunofluorescence: 1 µg/10⁶ cells.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Immunisation with Human CCR4 peptide.
• Specificity: Recognizes Human CCR4. Other species not tested.
• Formulation: Phosphate buffered saline, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: C-C motif chemokine receptor 4
• Database Link:
  Entrez Gene 1233 Human
  UniProt ID P51679
• Background: CCR4 (CC chemokine receptor 4) belongs to the rhodopsin family of G-protein-coupled receptors. Chemokine receptors are membrane-bound molecules composed of 7-transmembrane domains and are coupled to G-proteins (1,2). CCR4 binds the chemokines CCL17 (TARC) and CCL22 (MDC) and is highly expressed in most single-positive CD4(+) thymocytes especially in TH2- and regulatory T-cells (3,4). It plays a central role in T cell migration to the thymus, T cell maturation and education. CCR4 is often up-regulated in inflammation and cause conformational changes that trigger intracellular signaling pathways (2,5).
• Synonyms: C-C chemokine receptor type 4, C-C CKR-4, CC-CKR-4, CCR-4, CMKBR4, K5-5
• Note: SDS-PAGE analysis: The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Figure.3).

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction