S100A4 Mouse Monoclonal Antibody [Clone ID: NJ-4F3]

CAT#: DM1222

S100A4 mouse monoclonal antibody, clone NJ-4F3, Purified

Product Images

SDS-PAGE analysis of purified NJ-4F3 Antibody : Lane 1: Molecular weight marker. Lane 2: 2 ug of purified NJ-4F3 antibody. Proteins were separated by CGE (Capillary Gel Electrophoresis, Agilent 2100 Bioanalyzer). Internal Control bands (240 kDa / 7 kDa / 4.5 kDa).

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  FACS analysis of BOSC23 cells using NJ-4F3 Antibody DM122:. BOSC23 cells were transiently transfected with anexpression vector encoding either S100A4 (red curve) or anirrelevant protein (Control transfectant: black curve). Binding of NJ-4F3 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with S100A4 trans-fected cells.

Specifications

Product Data

• Clone Name: NJ-4F3
• Applications: ELISA, FC, WB
• Recommended Dilution: Flow cytometry: 1.2 µg/10⁶ cells.
  ELISA: 1/200-1/400.
  ELISA (capture): Clone NJ-4F3-D1 as detection antibody.
  ELISA (detection): Clone NJ-5C6-A3 as capture antibody.
  Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Genetic immunisation with cDNA encoding S100A4.
  Selection: based on recognition of the complete native protein expressed on transfected mammalian cells.
• Specificity: Recognizes Human S100 Calcium Binding Protein A4 (S100A4). Other species not tested.
• Formulation: Phosphate buffered saline, pH 7.2
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: S100 calcium binding protein A4
• Database Link:
  Entrez Gene 6275 Human
  UniProt ID P26447
• Background: S100 calcium binding protein A4 (S100A4) is a member of the S100 family of calcium-binding proteins that contain two Ca(2+)-binding sites including a canonical EF-hand motif. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells. S100A4 interacts with cytoskeletal proteins and enhances metastasis of several types of cancer cells. It is secreted by unknown mechanisms, thus, paracrinely stimulating a variety of cellular responses, including angiogenesis and neuronal growth (1). S100A4 has been shown to be a prognostic marker in a number of human cancers, including esophageal-squamous cancers, non-small lung cancers, primary gastric cancers, malignant melanomas, prostate cancers, bladder cancers, and pancreatic carcinomas. The universality of S100A4 expression in a variety of cancers illustrates the potential use of S100A4 as a marker for tumor metastasis and disease progression (2).
• Synonyms: S100-A4, CAPL, MTS1, Calvasculin, Metastasin, FSP-1, FSP1, Fibroblast marker, Fibroblast specific protein-1