CD66c/e Mouse Monoclonal Antibody [Clone ID: MUS]

CAT#: DM1203

CD66c/e mouse monoclonal antibody, clone MUS, Purified

Product Images

Fig.1: FACS analysis of BOSC23 cells using MUS Cat.# DM1203. BOSC23 cells were transiently transfected with anexpression vector encoding either CEACAM5 (red curve) oran irrelevant protein (control transfectant). Binding of MUSwas detected with a PE conjuga

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  Fig.2: FACS analysis of BOSC23 cells using MUS DM1203. BOSC23 cells were transiently transfected with anexpression vector encoding either CEACAM6 (red curve) oran irrelevant protein (control transfectant). Binding of MUSwas detected with a PE conjugated se

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  BOSC cells were transiently transfected with expression vectors containing eitherthe cDNA of CEACAM1, 3, 5, 6, 7, 8 or a recombinant transmembrane-anchored PSG1 fusion protein. Recognition ofCEACAM4 was tested on CHO cells stably transfected with a CEACAM4

Specifications

Product Data

• Clone Name: MUS
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: Flow Cytometry: 1.2 µg/10⁶ cells.
  ELISA: 1/200-1/400.
  Cell based ELISA with intakt, transiently transfected cells: 1/200.
  Western blot: 4 µg/ml.
  Immunohistochemistry: 1-2 µg/10⁶ cells (on Cryosections).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: MUS was generated by immunisation of BALB/c mice with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors (3).
• Specificity: This antibody reacts to CD66c/e.
• Formulation: Phosphate buffered saline, pH 7.2
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker (2). CEACAM6 expression is strongly upregulated already during early stages of adenocarcinoma formation (3). The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria (1).
• Synonyms: CEACAM5, Carcinoembryonic antigen, CEA, Meconium Antigen 100, Normal Cross-reacting Antigen, CEACAM6, NCA