Glycophorin A (GYPA) Mouse Monoclonal Antibody [Clone ID: JC159]

CAT#: DM066F

Glycophorin A (GYPA) mouse monoclonal antibody, clone JC159, FITC

Product Images

Surface staining of CD235a in human peripheral blood (erythrocytes and leukocytes) with anti-CD235a (JC159) FITC.

Specifications

Product Data

• Clone Name: JC159
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (0.4 ml) is sufficient for 100 tests.
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Membrane preparation from splenic hairy cell leukemia
• Specificity: This Monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.
• Formulation: Stabilizing Phosphate buffered saline (PBS)
  Label: FITC
  State: Liquid purified Ig fraction
  Preservative: 15 mM Sodium Azide
  Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: glycophorin A (MNS blood group)
• Database Link:
  Entrez Gene 2993 Human
  UniProt ID P02724
• Background: CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.
• Synonyms: Glycophorin-A, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein