IRAK4 Mouse Monoclonal Antibody [Clone ID: 6F8]

CAT#: DDX0340A546-100

IRAK4 mouse monoclonal antibody, clone 6F8

Product Images

IHC staining of human FFPE tonsil section (Vermi William courtesy)

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  IF staining of human tonsil with 6F8 (DDX0340)

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  WB with 6F8 (Innateplateform, CLARA, Lyon)

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  FACS staining of caninecell line with 6F8 (BonnefontC, personalcomm., 2011)

Specifications

Product Data

• Clone Name: 6F8
• Applications: FC, IF
• Recommended Dilution: DDX0340P-50 / DDX0340P-100 Purified: IHC paraffin, IF, Intracellular flow cytometry, WB.
  DDX0340A488-50 / DDX0340A488-100 Alexa-fluor®488: IF, Intracellular flow cytometry.
  DDX0340A546-50 / DDX0340A546-100 Alexa-fluor®546: IF, Intracellular flow cytometry.
  DDX0340A647-50 / DDX0340A647-100 Alexa-fluor®647: IF, Intracellular flow cytometry.
  
  Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use.
• Reactivities: Canine, Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: human recombinant IRAK-4 transfected 293T cells.
• Specificity: Human IRAK-4.
  Species cross-reactivity: Dog.
• Formulation: Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Alexa Fluor 546
• Concentration: lot specific
• Conjugation: Alexa Fluor 546
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: interleukin 1 receptor associated kinase 4
• Database Link:
  Entrez Gene 51135 Human
  UniProt ID Q9NWZ3
• Background: IL-1R associated kinases (IRAKs) are important mediators in the signal transduction of Toll / IL-1R (TIR) family members. The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK-M and IRAK4). IL-1 stimulation leads to the recruitment of the IL-1R associated kinase (IRAK) to the IL-1 Receptor where IRAK is phosphorylated, ubiquinated, and eventually degraded. Endogenous IRAK-4 interacts with IRAK-1 and TRAF6 in an IL-1 dependent manner. IRAK-4 is able to phosphorylate IRAK-1. The 6F8 clone was generated after mouse immunization with human IRAK4-transfected eukaryotic cells. This monoclonal antibody was demonstrated to cross-react with canine IRAK-4. (Cao S. et al, 1996 ; Science, 271, 1128-1131 ; Li S. et al, 2002 ; PNAS, 99, 5567-5572, Bonnefont C, personal communication, 2011).
• Synonyms: IRAK-4, NY-REN-64