Cd8a Rat Monoclonal Antibody [Clone ID: 53.6.72(lyt2)]

CAT#: DDX0091P-100

Cd8a rat monoclonal antibody, clone 53.6.72(lyt2)

Product Images

FACS staining: expression on JY transfected cells.

Specifications

Product Data

• Clone Name: 53.6.72(lyt2)
• Applications: FC
• Recommended Dilution: Flow Cytometry: This monoclonal antibody may be used between 1-10 μg/ml.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: thymic and spleen mouse cells
• Specificity: Mouse CD8α (Lyt-2).
• Formulation: State: Purified
  State: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Unconjugated
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
• Gene Name: CD8 antigen, alpha chain
• Database Link:
  Entrez Gene 12525 Mouse
  UniProt ID P01731
• Background: CD8 exists in two forms: a CD8 heterodimer composed of a α chain and a ß chain; and a homodimer of two α chains (CD8α = Lyt2, Ly2, OX8). The heterodimer is found on essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC-Class I-restricted CD8+ T cells. CD8 is required for the development of cytotoxic T cells.
  (Ledbetter JA et al, 1979, Nat. Rev. Immunol., 47, 63: 425-30)
• Synonyms: CD8 alpha chain, CD8A, MAL