Thy1 Mouse Monoclonal Antibody [Clone ID: T11D7e]

CAT#: CL037PS

Thy1 mouse monoclonal antibody, clone T11D7e, Purified

Specifications

Product Data

• Clone Name: T11D7e
• Applications: CT, FC
• Recommended Dilution: Cytotoxic Assays.
  Flow Cytometry: Use 10 µl of 1/5 diluted antibody to label 10⁶ cells in 100 µl.
  This product is routinely tested in Flow Cytometry using Mouse thymocytes.
• Reactivities: Mouse, Rat
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Xenogeneic rat thymocytes.
  Spleen cells from immunised CBA mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody recognises Mouse and Rat CD90 also known as Thy1.1. Thy1.1 is a monomorphic determinant in rat but polymorphic in mice.
  Clone T11D7e reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c.
  Clone T11D7e is useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgM fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Gene Name: thymus cell antigen 1, theta
• Database Link:
  Entrez Gene 21838 Mouse
  UniProt ID P01831
• Background: CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1
• Synonyms: Thy-1, THY1, CDw90