Cd8a Rat Monoclonal Antibody [Clone ID: YTS169.4]

CAT#: CL007P

Cd8a rat monoclonal antibody, clone YTS169.4, Purified

Product Images

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD8 antibody, clone YTS169.4 Cat.-No CL007P) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody. Medium power.

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  Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD8 antibody, clone YTS169.4 (Cat.-No CL007P) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody. Low power.

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  Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD8 antibody, clone YTS169.4 (Cat.-No CL007P) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody. High power.

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  CL007P (RAT ANTI MOUSE CD8) staining of mouse peripheral blood lymphocytes visualised with F (ab')2 RABBIT ANTI RAT IgG: FITC.

Specifications

Product Data

• Clone Name: YTS169.4
• Applications: FC, IHC
• Recommended Dilution: Flow cytometry: 1/50-1/100, use 10 µl to label 10⁶ cells in 100 µl. Method sheets are available on request.
  Immunohistochemistry on Frozen Sections.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Specificity: This clone recognises the Murine CD8 cell surface antigen, expressed by a subset of T lymphocytes.
  Clone YTS169.4 exhibits depleting activity when used in vivo.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD8 antigen, alpha chain
• Database Link:
  Entrez Gene 12525 Mouse
  UniProt ID P01731
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL