Nestin (NES) Mouse Monoclonal Antibody [Clone ID: OTI7D9]

CAT#: CF814262

Carrier-free (BSA/glycerol-free) Nestin(NES) mouse monoclonal antibody,clone OTI7D9

Product Images

IHC staining of FFPE human colon tissue with in the normal limits using anti-Nestin(NES) mouse monoclonal antibody (TA814262) and Polink-2 HRP polymer detection kit (D22-110). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 (B04C-100) at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.

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  IHC staining of FFPE human kidney tissue with in the normal limits using anti-Nestin(NES) mouse monoclonal antibody (TA814262) and Polink-2 HRP polymer detection kit (D22-110). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 (B04C-100) at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.

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  IHC staining of FFPE human thyroid cancer tissue using anti-Nestin(NES) mouse monoclonal antibody (TA814262) and Polink-2 HRP polymer detection kit (D22-110). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 (B04C-100) at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.

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  IHC staining of FFPE human glioma tissue using anti-Nestin(NES) mouse monoclonal antibody (TA814262) and Polink-2 HRP polymer detection kit (D22-110). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 (B04C-100) at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.

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  IHC staining of FFPE human appendix tissue with in the normal limits using anti-Nestin(NES) mouse monoclonal antibody (TA814262) and Polink-2 HRP polymer detection kit (D22-110). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 (B04C-100) at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.

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  IHC staining of FFPE human muscle tissue with in the normal limits using anti-Nestin(NES) mouse monoclonal antibody (TA814262) and Polink-2 HRP polymer detection kit (D22-110). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 (B04C-100) at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.

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Specifications

Product Data

• Clone Name: OTI7D9
• Applications: IHC
• Recommended Dilution: IHC 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment of human Nestin(NES) (NP_006608) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Concentration: 1mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 177.3 kDa
• Gene Name: nestin
• Database Link:
  NP_006608
  Entrez Gene 10763 Human
  UniProt ID P48681
• Background: This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]
• Synonyms: Nbla00170

Reference Data

• Protein Families: ES Cell Differentiation/IPS