Perforin (PRF1) Mouse Monoclonal Antibody [Clone ID: OTI3D7]
CAT#: CF814190
Carrier-free (BSA/glycerol-free) Perforin-1 mouse monoclonal antibody, clone OTI3D7
Formulation: Standard
Specifications
Product Data | |
Clone Name | OTI3D7 |
Applications | IHC |
Recommended Dilution | IHC 1:100 |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Human recombinant protein fragment corresponding to amino acids 351-555 of human Perforin-1 (NP_005032) produced in E.coli. |
Formulation | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
Conjugation | Unconjugated |
Storage | Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws. |
Stability | Stable for 12 months from date of receipt. |
Predicted Protein Size | 61.38 kDa |
Gene Name | perforin 1 |
Database Link | |
Background | This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017] |
Synonyms | HPLH2; P1; PFP |
Reference Data | |
Protein Families | Druggable Genome |
Protein Pathways | Allograft rejection, Autoimmune thyroid disease, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Antibody Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
TA814190 | Perforin-1 mouse monoclonal antibody, clone OTI3D7 |
USD 447.00 |
|
TA814190AM | Perforin-1 mouse monoclonal antibody, clone OTI3D7, Biotinylated |
USD 509.00 |
|
TA814190BM | Perforin-1 mouse monoclonal antibody, clone OTI3D7, HRP conjugated |
USD 509.00 |
|
TA814190S | Perforin-1 mouse monoclonal antibody, clone OTI3D7 |
USD 200.00 |
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