CD47 Mouse Monoclonal Antibody [Clone ID: OTI3H1]

CAT#: CF813270

Carrier-free (BSA/glycerol-free) CD47 mouse monoclonal antibody,clone OTI3H1

Product Images

Flow cytometric analysis of living 293T cells transfected with CD47 overexpression plasmid (RC218813), Red) using anti-CD47 antibody (TA813270). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

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  Flow cytometric analysis of living Jurkat cells, using anti-CD47 antibody (TA813270, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

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  Flow cytometric analysis of living Raji cells, using anti-CD47 antibody (TA813270, Red), compared to an isotype control (green) (1:100).

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  Flow cytometric analysis of living Ramos cells, using anti-CD47 antibody (TA813270, Red), compared to an isotype control (green) (1:100).

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  Flow cytometric analysis of living K562 cells, using anti-CD47 antibody (TA813270, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

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  Flow cytometric analysis of living PBMCs, using anti-CD47 antibody (TA813270, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

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Specifications

Product Data

• Clone Name: OTI3H1
• Applications: FC
• Recommended Dilution: FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD47 (NP_001768) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.21 kDa
• Gene Name: CD47 molecule
• Database Link:
  NP_001768
  Entrez Gene 961 Human
  UniProt ID Q08722
• Background: This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]
• Synonyms: IAP; MER6; OA3

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ECM-receptor interaction